Suchergebnisse

Freshwater mussels are a critically imperiled group of mollusks that play key ecological roles and provide important services to humans. The Ambleminae is the only subfamily of these mussels, endemic to North America. Complete mitogenomes have only been sequenced for two of five tribes of the subfamily. Pleurobema oviforme, Amblema plicata, and Popenaias popeii each belong to tribes Pleurobemini, Amblemini, and Popenaidini, respectively, and have not had published mitogenomes. Thus, this study aims to present the complete mitogenomes for these species, to provide a phylogeny of the Ambleminae and confirm the gene arrangements with representation from each of its tribes. The newly sequenced mitogenomes range from 15,852 to 15,993 nucleotides, are composed of 13 PCGs, 22 tRNAs, and two rRNAs and all share the same (UF1) gene order. ; This work was supported by Portuguese Foundation for Science and Technology (FCT) [grant number ConBioMics/BI-Lic/2019-037 (JTT), grant number SFRH/BD/137935/2018 (AGS)]; COMPETE 2020, Portugal 2020 and the European Union through the ERDF, and by Portuguese Foundation for Science and Technology (FCT) through national funds [UID/Multi/04423/2019] under project ConBiomics: the missing approach for the Conservation of Bivalves Project, and [project number NORTE-01- 0145-FEDER-030286]. Fieldwork in Texas was funded by the U.S. Fish and Wildlife Service, and Texas Parks and Wildlife Department (TPWD) as a Joint Traditional Section 6 Project 407348. ; info:eu-repo/semantics/publishedVersion

Since historical times, the inherent human fascination with pearls turned the freshwater pearl mussel Margaritifera margaritifera (Linnaeus, 1758) into a highly valuable cultural and economic resource. Although pearl harvesting in M. margaritifera is nowadays residual, other human threats have aggravated the species conservation status, especially in Europe. This mussel presents a myriad of rare biological features, e.g. high longevity coupled with low senescence and Doubly Uniparental Inheritance of mitochondrial DNA, for which the underlying molecular mechanisms are poorly known. Here, the first draft genome assembly of M. margaritifera was produced using a combination of Illumina Paired-end and Mate-pair approaches. The genome assembly was 2.4 Gb long, possessing 105,185 scaffolds and a scaffold N50 length of 288,726 bp. The ab initio gene prediction allowed the identification of 35,119 protein-coding genes. This genome represents an essential resource for studying this species' unique biological and evolutionary features and ultimately will help to develop new tools to promote its conservation. ; A.G.-d.-S. was funded by the Portuguese Foundation for Science and Technology (FCT) under the grants SFRH/BD/137935/2018, EF (CEECIND/00627/2017) and MLL (2020.03608.CEECIND). This research was developed under ConBiomics: the missing approach for the Conservation of freshwater Bivalves Project No. NORTE-01-0145-FEDER- 030286, co-financed by COMPETE 2020, Portugal 2020 and the European Union through the ERDF, and by FCT through national funds. Additional strategic funding was provided by FCT UIDB/04423/2020 and UIDP/04423/2020. Authors' interaction and writing of the article was promoted and facilitated by the COST Action CA18239: CONFREMU—Conservation of freshwater mussels: a pan- European approach. ; info:eu-repo/semantics/publishedVersion

The complete mitogenomes of one (M-)ale (North America), one Hermaphroditic (Europe), and two (F-)emale (North America and Europe) individuals of the freshwater pearl mussel Margaritifera margaritifera were sequenced. The M-type and F-type (Female and Hermaphroditic) mitogenomes have 17,421 and 16,122 nucleotides, respectively. All with the same content: 13 protein-coding genes, 22 transfer RNA, two ribosomal RNA genes, and one sex-related ORF. The M-type is highly divergent (37.6% uncorrected p-distance) from the F-type mitogenomes. North American and European F-type mitogenomes exhibit low genetic divergence (68 nt substitutions), and the Female and Hermaphroditic European mitogenomes are almost identical, and matching sex-related ORFs. ; This work was supported by Portuguese Foundation for Science and Technology (FCT) [grant number SFRH/BD/115728/2016 (MLL), grant number SFRH/BD/137935/2018 (AGS)]; Russian Foundation for Basic Research [grant number 18-34-20033 (IVV)]; Dawson Fellowship at St. Catharine's College, Cambridge (DCA); Life Margal Ulla [number LIFE09 NAT/ES/000514 (RA and PO)]; COMPETE 2020, Portugal 2020 and the European Union through the ERDF, and by Portuguese Foundation for Science and Technology (FCT) through national funds [UID/Multi/04423/2019] under project ConBiomics: the missing approach for the Conservation of freshwater Bivalves Project, and [project number NORTE-01-0145-FEDER-030286]; Federal Agency for Scientific Organizations under Grants [grant number 0409-2015-0143 (INB and IVV)] ; info:eu-repo/semantics/publishedVersion

The complete mitogenomes of one (M-)ale (North America), one Hermaphroditic (Europe), and two (F-)emale (North America and Europe) individuals of the freshwater pearl mussel Margaritifera margaritifera were sequenced. The M-type and F-type (Female and Hermaphroditic) mitogenomes have 17,421 and 16,122 nucleotides, respectively. All with the same content: 13 protein-coding genes, 22 transfer RNA, two ribosomal RNA genes, and one sex-related ORF. The M-type is highly divergent (37.6% uncorrected p-distance) from the F-type mitogenomes. North American and European F-type mitogenomes exhibit low genetic divergence (68 nt substitutions), and the Female and Hermaphroditic European mitogenomes are almost identical, and matching sex-related ORFs. ; This work was supported by Portuguese Foundation for Science and Technology (FCT) [grant number SFRH/BD/115728/2016 (MLL), grant number SFRH/BD/137935/2018 (AGS)]; Russian Foundation for Basic Research [grant number 18-34-20033 (IVV)]; Dawson Fellowship at St. Catharine's College, Cambridge (DCA); Life Margal Ulla [number LIFE09 NAT/ES/000514 (RA and PO)]; COMPETE 2020, Portugal 2020 and the European Union through the ERDF, and by Portuguese Foundation for Science and Technology (FCT) through national funds [UID/Multi/04423/2019] under project ConBiomics: the missing approach for the Conservation of freshwater Bivalves Project, and [project number NORTE-01-0145-FEDER-030286]; Federal Agency for Scientific Organizations under Grants [grant number 0409-2015-0143 (INB and ...

Anthropogenic freshwater habitats may provide undervalued prospects for long-term conservation as part of species conservation planning. This fundamental, but overlooked, issue requires attention considering the pace that humans have been altering natural freshwater ecosystems and the accelerated levels of biodiversity decline in recent decades. We compiled 709 records of freshwater mussels (Bivalvia, Unionida) inhabiting a broad variety of anthropogenic habitat types (from small ponds to large reservoirs and canals) and reviewed their importance as refuges for this faunal group. Most records came from Europe and North America, with a clear dominance of canals and reservoirs. The dataset covered 228 species, including 34 threatened species on the IUCN Red List. We discuss the conservation importance and provide guidance on how these anthropogenic habitats could be managed to provide optimal conservation value to freshwater mussels. This review also shows that some of these habitats may function as ecological traps owing to conflicting management practices or because they act as a sink for some populations. Therefore, anthropogenic habitats should not be seen as a panacea to resolve conservation problems. More information is necessary to better understand the trade-offs between human use and the conservation of freshwater mussels (and other biota) within anthropogenic habitats, given the low number of quantitative studies and the strong biogeographic knowledge bias that persists. ; This publication is based upon work from COST Action CA18239, supported by COST (European Cooperation in Science and Technology). A.M.L. was financed by the Institute of Environmental Sciences Jagiellonian University (N18/DBS/000003) and K.N. by the Aragón Government. The authors acknowledge Jarosław Andrzejewski, Bartosz Czader, Anna Fica, Marcin Horbacz, Tomasz Jonderko, Steinar Kålås, Tomasz Kapela, Bjørn Mejdell Larsen, Maciej Pabijan, Katarzyna Pawlik, Ilona Popławska, Joanna Przybylska, Tomasz Przybył, Mateusz Rybak, Kjell ...